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The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence
(Oxford University Press, 1998-01-15)
Telomerase is a ribonucleoprotein enzyme with an essential RNA component. Embedded within the telomerase RNA is a template sequence for telomere synthesis. We have characterized the structure of the 5' regions of the human ...
Identification of a Specific Telomere Terminal Transferase Activity in Tetrahymena Extracts
(Cell Press, 1985-12)
We have found a novel activity in Tetrahymena cell free extracts that adds tandem TTGGGG repeats onto synthetic telomere primers. The single-stranded DNA oligonucleotides (TTGGGG)4 and TGTGTGGGTGTGTGGGTGTGTGGG, consisting ...
Telomere dysfunction triggers developmentally regulated germ cell apoptosis
(American Society for Cell Biology, 2001-07)
Telomere dysfunction results in fertility defects in a number of organisms. Although data from fission yeast and Caenorhabditis elegans suggests that telomere dysfunction manifests itself primarily as defects in proper ...
Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme
(Cell Press, 1995-06-02)
Telomerase is a ribonucleoprotein DNA polymerase that catalyzes the de novo synthesis of telomeric simple sequence repeats. We describe the purification of telomerase and the cloning of cDNAs encoding two protein subunits ...
Functional reconstitution of wild-type and mutant Tetrahymena telomerase
(Cold Spring Harbor Laboratory Press, 1994-03-01)
Telomerase is a ribonucleoprotein that catalyzes telomere elongation in vitro and in vivo. The 159-nucleotide RNA component of Tetrahymena telomerase contains the sequence 5'-CAACCCCAA-3' ("template region"), which serves ...
Mutational analysis of the Tetrahymena telomerase RNA: identification of residues affecting telomerase activity in vitro
(Oxford University Press, 1998-02-01)
Telomere-specific repeat sequences are essential for chromosome end stability. Telomerase maintains telomere length by adding sequences de novo onto chromosome ends. The template domain of the telomerase RNA component ...
The Telomere Terminal Transferase of Tetrahymena Is a Ribonucleoprotein Enzyme with Two Kinds of Primer Specificity
(Cell Press, 1987-12-24)
We have analyzed the de novo telomere synthesis catalyzed by the enzyme telomere terminal transferase (telomerase) from Tetrahymena. Oligonucleotides representing the G-rich strand of telomeric sequences from five different ...
Identification of two RNA-binding proteins associated with human telomerase RNA
(American Society for Cell Biology, 2000-03)
Telomerase plays a crucial role in telomere maintenance in vivo. To understand telomerase regulation, we have been characterizing components of the enzyme. To date several components of the mammalian telomerase holoenzyme ...
Telomere Dysfunction Increases Mutation Rate and Genomic Instability
(Cell Press, 2001-08-10)
The increased tumor incidence in telomerase null mice suggests that telomere dysfunction induces genetic instability. To test this directly, we examined mutation rate in the absence of telomerase in S. cerevisiae. The ...
G-strand overhangs on telomeres in telomerase-deficient mouse cells
(Oxford University Press, 1999-10-15)
Telomeres of eukaryotic chromosomes contain 3' overhangs which are thought to be essential for the maintenance of proper chromosome end structure and function. We examined the requirement for telomerase activity for the ...