Now showing items 1-10 of 30
The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence
(Oxford University Press, 1998-01-15)
Telomerase is a ribonucleoprotein enzyme with an essential RNA component. Embedded within the telomerase RNA is a template sequence for telomere synthesis. We have characterized the structure of the 5' regions of the human ...
Identification of a Specific Telomere Terminal Transferase Activity in Tetrahymena Extracts
(Cell Press, 1985-12)
We have found a novel activity in Tetrahymena cell free extracts that adds tandem TTGGGG repeats onto synthetic telomere primers. The single-stranded DNA oligonucleotides (TTGGGG)4 and TGTGTGGGTGTGTGGGTGTGTGGG, consisting ...
Telomere dysfunction triggers developmentally regulated germ cell apoptosis
(American Society for Cell Biology, 2001-07)
Telomere dysfunction results in fertility defects in a number of organisms. Although data from fission yeast and Caenorhabditis elegans suggests that telomere dysfunction manifests itself primarily as defects in proper ...
Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme
(Cell Press, 1995-06-02)
Telomerase is a ribonucleoprotein DNA polymerase that catalyzes the de novo synthesis of telomeric simple sequence repeats. We describe the purification of telomerase and the cloning of cDNAs encoding two protein subunits ...
Longevity, Stress Response, and Cancer in Aging Telomerase-Deficient Mice
(Cell Press, 1999-03-05)
Telomere maintenance is thought to play a role in signaling cellular senescence; however, a link with organismal aging processes has not been established. The telomerase null mouse provides an opportunity to understand the ...
Short Telomeres, even in the Presence of Telomerase, Limit Tissue Renewal Capacity
(Cell Press, 2005-12-16)
Autosomal-dominant dyskeratosis congenita is associated with heterozygous mutations in telomerase. To examine the dosage effect of telomerase, we generated a line of mTR+/ÿ mice on the CAST/EiJ background, which has short ...
Telomere Shortening and Tumor Formation by Mouse Cells Lacking Telomerase RNA
(Cell Press, 1997-10-03)
To examine the role of telomerase in normal and neoplastic growth, the telomerase RNA component (mTR) was deleted from the mouse germline. mTR-/- mice lacked detectable telomerase activity yet were viable for the six ...
The Shortest Telomere, Not Average Telomere Length, Is Critical for Cell Viability and Chromosome Stability
(Cell Press, 2001-10-05)
Loss of telomere function can induce cell cycle arrest and apoptosis. To investigate the processes that trig- ger cellular responses to telomere dysfunction, we crossed mTR/ G6 mice that have short telomeres with mice ...
Functional reconstitution of wild-type and mutant Tetrahymena telomerase
(Cold Spring Harbor Laboratory Press, 1994-03-01)
Telomerase is a ribonucleoprotein that catalyzes telomere elongation in vitro and in vivo. The 159-nucleotide RNA component of Tetrahymena telomerase contains the sequence 5'-CAACCCCAA-3' ("template region"), which serves ...