Now showing items 7-26 of 31

    • Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues 

      Shay, Jerry W.; Avilion, Ariel A.; Piatyszek, Mieczyslaw A.; Gupta, Jyothi; Greider, Carol W.; Bacchetti, Silvia (American Association of Cancer Research, 1996-02-01)
      Telomerase activity has been detected in many human immortal cells lines and in tumor tissues, whereas it is generally absent from primary cell strains and from many tumor adjacent tissue samples. With the recently cloned ...
    • Identification of a Specific Telomere Terminal Transferase Activity in Tetrahymena Extracts 

      Blackburn, Elizabeth H.; Greider, Carol W. (Cell Press, 1985-12)
      We have found a novel activity in Tetrahymena cell free extracts that adds tandem TTGGGG repeats onto synthetic telomere primers. The single-stranded DNA oligonucleotides (TTGGGG)4 and TGTGTGGGTGTGTGGGTGTGTGGG, consisting ...
    • Identification of two RNA-binding proteins associated with human telomerase RNA 

      Greider, Carol W.; Sternglanz, Rolf; Le, Siyuan (American Society for Cell Biology, 2000-03)
      Telomerase plays a crucial role in telomere maintenance in vivo. To understand telomerase regulation, we have been characterizing components of the enzyme. To date several components of the mammalian telomerase holoenzyme ...
    • Longevity, Stress Response, and Cancer in Aging Telomerase-Deficient Mice 

      DePinho, Ronald A.; Greider, Carol W.; Gottlieb, Geoffrey J.; Blasco, Maria; Lee, Han-Woong; Chang, Sandy; Rudolph, Karl Lenhard (Cell Press, 1999-03-05)
      Telomere maintenance is thought to play a role in signaling cellular senescence; however, a link with organismal aging processes has not been established. The telomerase null mouse provides an opportunity to understand the ...
    • The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence 

      Herr, Winship; Greider, Carol W.; Villeponteau, Bryant; Feng, Junli; Funk, Walter D.; Blasco, Maria A.; Hinckley, Craig S. (Oxford University Press, 1998-01-15)
      Telomerase is a ribonucleoprotein enzyme with an essential RNA component. Embedded within the telomerase RNA is a template sequence for telomere synthesis. We have characterized the structure of the 5' regions of the human ...
    • Mutational analysis of the Tetrahymena telomerase RNA: identification of residues affecting telomerase activity in vitro 

      Greider, Carol W.; Autexier, Chantal (Oxford University Press, 1998-02-01)
      Telomere-specific repeat sequences are essential for chromosome end stability. Telomerase maintains telomere length by adding sequences de novo onto chromosome ends. The template domain of the telomerase RNA component ...
    • p53 Deficiency Rescues the Adverse Effects of Telomere Loss and Cooperates with Telomere Dysfunction to Accelerate Carcinogenesis 

      DePinho, Ronald A.; Greider, Carol W.; Gottlieb, Geoffrey J.; Lee, Shwu-Luan; Tam, Alice; Shen, Qiong; Artandi, Steven E.; Chin, Lynda (Cell Press, 1999-05-14)
      Maintenance of telomere length and function is critical for the efficient proliferation of eukaryotic cells. Here, we examine the interactions between telomere dysfunction and p53 in cells and organs of telomerase-deficient ...
    • Phosphorylation of H2AX at short telomeres in T cells and fibroblasts 

      Greider, Carol W.; Strong, Margaret A.; Hao, Ling-Yang (American Society for Biochemistry and Molecular Biology, 2004-10-22)
      Eukaryotic cells undergo arrest and enter apoptosis in response to short telomeres. T cells from late generation mTR(-/-) mice that lack telomerase show increased apoptosis when stimulated to enter the cell cycle. The ...
    • Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme 

      Greider, Carol W.; Kobayashi, Ryuji; Collins, Kathleen (Cell Press, 1995-06-02)
      Telomerase is a ribonucleoprotein DNA polymerase that catalyzes the de novo synthesis of telomeric simple sequence repeats. We describe the purification of telomerase and the cloning of cDNAs encoding two protein subunits ...
    • The RNA component of human telomerase 

      Greider, Carol W.; Feng, Junli (American Association for the Advancement of Science, 1995-09-01)
      Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA)-protein complex. Here, the ...
    • Secondary structure of vertebrate telomerase RNA 

      Greider, Carol W.; Blasco, Maria A.; Chen, Jiunn-Liang (Cell Press, 2000-03-03)
      Telomerase is a ribonucleoprotein enzyme that maintains telomere length by adding telomeric sequence repeats onto chromosome ends. The essential RNA component of telomerase provides the template for telomeric repeat ...
    • Short dysfunctional telomeres impair tumorigenesis in the INK4a(delta2/3) cancer-prone mouse. 

      DePinho, Ronald A.; Greider, Carol W.; Singer, Robert H.; Gottlieb, Geoffrey J.; Lee, Kee-Ho; Femino, Andrea; Chin, Lynda; Greenberg, Roger A. (Cell Press, 1999-05-14)
      Maintenance of telomere length is predicted to be essential for bypass of senescence and crisis checkpoints in cancer cells. The impact of telomere dysfunction on tumorigenesis was assessed in successive generations of ...
    • Short telomeres and ataxia-telangiectasia mutated deficiency cooperatively increase telomere dysfunction and suppress tumorigenesis 

      Armanios, Mary; Qi, Ling; Strong, Margaret A.; Karim, Baktiar O.; Greider, Carol W.; Huso, David L. (American Association of Cancer Research, 2003-12-01)
      To examine the role of ataxia-telangiectasia mutated (Atm) in telomere function, we generated Atm and telomerase null mice (Atm(-/-) mTR(-/-) iG6 mice). These mice exhibited increased germ cell death and chromosome fusions ...
    • Short telomeres induce a DNA damage response in Saccharomyces cerevisiae 

      Greider, Carol W.; IJipma, Arne S. (American Society for Cell Biology, 2003-03)
      Telomerase-deficient Saccharomyces cerevisiae cells show a progressive decrease in telomere length. When grown for several days in log phase, the tlc1Delta cells initially display wild-type growth kinetics with subsequent ...
    • Short Telomeres Limit Tumor Progression In Vivo by Inducing Senescence 

      Greider, Carol W.; Feldser, David M. (Cell Press, 2007-05)
      Telomere maintenance is critical for cancer progression. To examine mechanisms of tumor suppression induced by short telomeres, we crossed mice deficient for the RNA component of telomerase, mTR(-/-), with Emu-myc transgenic ...
    • Short Telomeres, even in the Presence of Telomerase, Limit Tissue Renewal Capacity 

      Greider, Carol W.; Huso, David; Feldser, David M.; Karim, Baktiar; Strong, Margaret A.; Armanios, Mary; Hao, Ling-Yang (Cell Press, 2005-12-16)
      Autosomal-dominant dyskeratosis congenita is associated with heterozygous mutations in telomerase. To examine the dosage effect of telomerase, we generated a line of mTR+/ÿ mice on the CAST/EiJ background, which has short ...
    • The Shortest Telomere, Not Average Telomere Length, Is Critical for Cell Viability and Chromosome Stability 

      Greider, Carol W.; Hao, Ling-Yang; Strong, Margaret A.; Hemann, Michael T. (Cell Press, 2001-10-05)
      Loss of telomere function can induce cell cycle arrest and apoptosis. To investigate the processes that trig- ger cellular responses to telomere dysfunction, we crossed mTR􏰀/􏰀 G6 mice that have short telomeres with mice ...
    • Telomerase mutations in families with idiopathic pulmonary fibrosis 

      Loyd, James E.; Greider, Carol W.; Lansdorp, Peter M.; Phillips, John A., III; Vulto, Irma; Xie, Mingyi; Lawson, William E.; Markin, Cheryl; Ingersoll, Roxann G.; Alder, Jonathan K.; Cogan, Joy D.; Chen, Julian J.-L.; Armanios, Mary Y. (Massachusetts Medical Society, 2007-03-29)
      BACKGROUND: Idiopathic pulmonary fibrosis is progressive and often fatal; causes of familial clustering of the disease are unknown. Germ-line mutations in the genes hTERT and hTR, encoding telomerase reverse transcriptase ...
    • Telomere Dysfunction Increases Mutation Rate and Genomic Instability 

      Greider, Carol W.; Feldser, David M.; Hackett, Jennifer A. (Cell Press, 2001-08-10)
      The increased tumor incidence in telomerase null mice suggests that telomere dysfunction induces genetic instability. To test this directly, we examined mutation rate in the absence of telomerase in S. cerevisiae. The ...
    • Telomere dysfunction triggers developmentally regulated germ cell apoptosis 

      Greider, Carol W.; Chin, Lynda; DePinho, Ronald A.; Strong, Margaret A.; Rudolph, Karl Lenhard; Hemann, Michael T. (American Society for Cell Biology, 2001-07)
      Telomere dysfunction results in fertility defects in a number of organisms. Although data from fission yeast and Caenorhabditis elegans suggests that telomere dysfunction manifests itself primarily as defects in proper ...