Now showing items 1-20 of 31

    • Identification of a Specific Telomere Terminal Transferase Activity in Tetrahymena Extracts 

      Blackburn, Elizabeth H.; Greider, Carol W. (Cell Press, 1985-12)
      We have found a novel activity in Tetrahymena cell free extracts that adds tandem TTGGGG repeats onto synthetic telomere primers. The single-stranded DNA oligonucleotides (TTGGGG)4 and TGTGTGGGTGTGTGGGTGTGTGGG, consisting ...
    • The Telomere Terminal Transferase of Tetrahymena Is a Ribonucleoprotein Enzyme with Two Kinds of Primer Specificity 

      Blackburn, Elizabeth H.; Greider, Carol W. (Cell Press, 1987-12-24)
      We have analyzed the de novo telomere synthesis catalyzed by the enzyme telomere terminal transferase (telomerase) from Tetrahymena. Oligonucleotides representing the G-rich strand of telomeric sequences from five different ...
    • Chromosome first aid 

      Greider, Carol W. (Cell Press, 1991-11-15)
    • Functional reconstitution of wild-type and mutant Tetrahymena telomerase 

      Greider, Carol W.; Autexier, Chantal (Cold Spring Harbor Laboratory Press, 1994-03-01)
      Telomerase is a ribonucleoprotein that catalyzes telomere elongation in vitro and in vivo. The 159-nucleotide RNA component of Tetrahymena telomerase contains the sequence 5'-CAACCCCAA-3' ("template region"), which serves ...
    • Gel shift and UV cross-linking analysis of Tetrahymena telomerase. 

      Greider, Carol; Crittenden, Jill; Hull, Christina; Harrington, Lea (American Society for Biochemistry and Molecular Biology, 1995-04-14)
      Telomerase is an unusual ribonucleoprotein that synthesizes new telomeres onto chromosome ends. The enzyme has been most extensively characterized in ciliates, where the RNA component has been cloned from several species, ...
    • Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme 

      Greider, Carol W.; Kobayashi, Ryuji; Collins, Kathleen (Cell Press, 1995-06-02)
      Telomerase is a ribonucleoprotein DNA polymerase that catalyzes the de novo synthesis of telomeric simple sequence repeats. We describe the purification of telomerase and the cloning of cDNAs encoding two protein subunits ...
    • The RNA component of human telomerase 

      Greider, Carol W.; Feng, Junli (American Association for the Advancement of Science, 1995-09-01)
      Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA)-protein complex. Here, the ...
    • Functional characterization and developmental regulation of mouse telomerase RNA 

      Greider, Carol W.; Villeponteau, Bryant; Funk, Walter; Blasco, Maria A. (American Association for the Advancement of Science, 1995-09-01)
      Telomerase synthesizes telomeric DNA repeats onto chromosome ends de novo. The mouse telomerase RNA component was cloned and contained only 65 percent sequence identity with the human telomerase RNA. Alteration of the ...
    • Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues 

      Shay, Jerry W.; Avilion, Ariel A.; Piatyszek, Mieczyslaw A.; Gupta, Jyothi; Greider, Carol W.; Bacchetti, Silvia (American Association of Cancer Research, 1996-02-01)
      Telomerase activity has been detected in many human immortal cells lines and in tumor tissues, whereas it is generally absent from primary cell strains and from many tumor adjacent tissue samples. With the recently cloned ...
    • Telomere Shortening and Tumor Formation by Mouse Cells Lacking Telomerase RNA 

      Greider, Carol W.; DePinho, Ronald A.; Lansdorp, Peter M.; Semper, Enrique; Hande, M. Prakash; Lee, Han-Woong; Blasco, Maria A. (Cell Press, 1997-10-03)
      To examine the role of telomerase in normal and neoplastic growth, the telomerase RNA component (mTR) was deleted from the mouse germline. mTR-/- mice lacked detectable telomerase activity yet were viable for the six ...
    • The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence 

      Herr, Winship; Greider, Carol W.; Villeponteau, Bryant; Feng, Junli; Funk, Walter D.; Blasco, Maria A.; Hinckley, Craig S. (Oxford University Press, 1998-01-15)
      Telomerase is a ribonucleoprotein enzyme with an essential RNA component. Embedded within the telomerase RNA is a template sequence for telomere synthesis. We have characterized the structure of the 5' regions of the human ...
    • Mutational analysis of the Tetrahymena telomerase RNA: identification of residues affecting telomerase activity in vitro 

      Greider, Carol W.; Autexier, Chantal (Oxford University Press, 1998-02-01)
      Telomere-specific repeat sequences are essential for chromosome end stability. Telomerase maintains telomere length by adding sequences de novo onto chromosome ends. The template domain of the telomerase RNA component ...
    • Longevity, Stress Response, and Cancer in Aging Telomerase-Deficient Mice 

      DePinho, Ronald A.; Greider, Carol W.; Gottlieb, Geoffrey J.; Blasco, Maria; Lee, Han-Woong; Chang, Sandy; Rudolph, Karl Lenhard (Cell Press, 1999-03-05)
      Telomere maintenance is thought to play a role in signaling cellular senescence; however, a link with organismal aging processes has not been established. The telomerase null mouse provides an opportunity to understand the ...
    • Telomeres Do D-Loop–T-Loop 

      Greider, Carol W. (Cell Press, 1999-05-14)
    • p53 Deficiency Rescues the Adverse Effects of Telomere Loss and Cooperates with Telomere Dysfunction to Accelerate Carcinogenesis 

      DePinho, Ronald A.; Greider, Carol W.; Gottlieb, Geoffrey J.; Lee, Shwu-Luan; Tam, Alice; Shen, Qiong; Artandi, Steven E.; Chin, Lynda (Cell Press, 1999-05-14)
      Maintenance of telomere length and function is critical for the efficient proliferation of eukaryotic cells. Here, we examine the interactions between telomere dysfunction and p53 in cells and organs of telomerase-deficient ...
    • Short dysfunctional telomeres impair tumorigenesis in the INK4a(delta2/3) cancer-prone mouse. 

      DePinho, Ronald A.; Greider, Carol W.; Singer, Robert H.; Gottlieb, Geoffrey J.; Lee, Kee-Ho; Femino, Andrea; Chin, Lynda; Greenberg, Roger A. (Cell Press, 1999-05-14)
      Maintenance of telomere length is predicted to be essential for bypass of senescence and crisis checkpoints in cancer cells. The impact of telomere dysfunction on tumorigenesis was assessed in successive generations of ...
    • G-strand overhangs on telomeres in telomerase-deficient mouse cells 

      Greider, Carol W.; Hemann, Michael T. (Oxford University Press, 1999-10-15)
      Telomeres of eukaryotic chromosomes contain 3' overhangs which are thought to be essential for the maintenance of proper chromosome end structure and function. We examined the requirement for telomerase activity for the ...
    • Identification of two RNA-binding proteins associated with human telomerase RNA 

      Greider, Carol W.; Sternglanz, Rolf; Le, Siyuan (American Society for Cell Biology, 2000-03)
      Telomerase plays a crucial role in telomere maintenance in vivo. To understand telomerase regulation, we have been characterizing components of the enzyme. To date several components of the mammalian telomerase holoenzyme ...
    • Secondary structure of vertebrate telomerase RNA 

      Greider, Carol W.; Blasco, Maria A.; Chen, Jiunn-Liang (Cell Press, 2000-03-03)
      Telomerase is a ribonucleoprotein enzyme that maintains telomere length by adding telomeric sequence repeats onto chromosome ends. The essential RNA component of telomerase provides the template for telomeric repeat ...
    • Wild-derived inbred mouse strains have short telomeres 

      Greider, Carol W.; Hemann, Michael T. (Oxford University Press, 2000-11-15)
      Telomere length and telomerase activity directly affect the replicative capacity of primary human cells. Some have suggested that telomere length influences organismal lifespan. We compared telomere length distributions ...