Now showing items 1-10 of 15
The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence
(Oxford University Press, 1998-01-15)
Telomerase is a ribonucleoprotein enzyme with an essential RNA component. Embedded within the telomerase RNA is a template sequence for telomere synthesis. We have characterized the structure of the 5' regions of the human ...
Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme
(Cell Press, 1995-06-02)
Telomerase is a ribonucleoprotein DNA polymerase that catalyzes the de novo synthesis of telomeric simple sequence repeats. We describe the purification of telomerase and the cloning of cDNAs encoding two protein subunits ...
Functional reconstitution of wild-type and mutant Tetrahymena telomerase
(Cold Spring Harbor Laboratory Press, 1994-03-01)
Telomerase is a ribonucleoprotein that catalyzes telomere elongation in vitro and in vivo. The 159-nucleotide RNA component of Tetrahymena telomerase contains the sequence 5'-CAACCCCAA-3' ("template region"), which serves ...
Mutational analysis of the Tetrahymena telomerase RNA: identification of residues affecting telomerase activity in vitro
(Oxford University Press, 1998-02-01)
Telomere-specific repeat sequences are essential for chromosome end stability. Telomerase maintains telomere length by adding sequences de novo onto chromosome ends. The template domain of the telomerase RNA component ...
G-strand overhangs on telomeres in telomerase-deficient mouse cells
(Oxford University Press, 1999-10-15)
Telomeres of eukaryotic chromosomes contain 3' overhangs which are thought to be essential for the maintenance of proper chromosome end structure and function. We examined the requirement for telomerase activity for the ...
Gel shift and UV cross-linking analysis of Tetrahymena telomerase.
(American Society for Biochemistry and Molecular Biology, 1995-04-14)
Telomerase is an unusual ribonucleoprotein that synthesizes new telomeres onto chromosome ends. The enzyme has been most extensively characterized in ciliates, where the RNA component has been cloned from several species, ...
Longevity, Stress Response, and Cancer in Aging Telomerase-Deficient Mice
(Cell Press, 1999-03-05)
Telomere maintenance is thought to play a role in signaling cellular senescence; however, a link with organismal aging processes has not been established. The telomerase null mouse provides an opportunity to understand the ...
Telomere Shortening and Tumor Formation by Mouse Cells Lacking Telomerase RNA
(Cell Press, 1997-10-03)
To examine the role of telomerase in normal and neoplastic growth, the telomerase RNA component (mTR) was deleted from the mouse germline. mTR-/- mice lacked detectable telomerase activity yet were viable for the six ...
Telomeres Do D-Loop–T-Loop
(Cell Press, 1999-05-14)
Short dysfunctional telomeres impair tumorigenesis in the INK4a(delta2/3) cancer-prone mouse.
(Cell Press, 1999-05-14)
Maintenance of telomere length is predicted to be essential for bypass of senescence and crisis checkpoints in cancer cells. The impact of telomere dysfunction on tumorigenesis was assessed in successive generations of ...