Phosphorylation of H2AX at short telomeres in T cells and fibroblasts

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dc.contributor.author Greider, Carol W.
dc.contributor.author Strong, Margaret A.
dc.contributor.author Hao, Ling-Yang
dc.date.accessioned 2009-11-05T18:09:50Z
dc.date.available 2009-11-05T18:09:50Z
dc.date.issued 2004-10-22
dc.identifier.citation First Published on August 17, 2004, doi: 10.1074/jbc.M403924200 October 22, 2004 The Journal of Biological Chemistry, 279, 45148-45154. en
dc.identifier.uri http://jhir.library.jhu.edu/handle/1774.2/33537
dc.description.abstract Eukaryotic cells undergo arrest and enter apoptosis in response to short telomeres. T cells from late generation mTR(-/-) mice that lack telomerase show increased apoptosis when stimulated to enter the cell cycle. The increased apoptosis was not inhibited by colcemid, indicating that the response did not result from breakage of dicentric chromosomes at mitosis. The damage response protein gamma-H2AX localized to telomeres in metaphases from T cells and fibroblasts from mTR(-/-) cells with short telomeres. These data suggest that the major mechanism for induction of apoptosis in late generation mTR(-/-) cells is independent of chromosome segregation and that loss of telomere function through progressive telomere shortening in the absence of telomerase leads to recognition of telomeres as DNA breaks. en
dc.description.provenance Submitted by David Reynolds (davidr@jhu.edu) on 2009-11-05T18:09:50Z No. of bitstreams: 1 JourBiolChem279.pdf: 571700 bytes, checksum: 8c5b7a352cd49344a51f6c71fa086c08 (MD5) en
dc.description.provenance Made available in DSpace on 2009-11-05T18:09:50Z (GMT). No. of bitstreams: 1 JourBiolChem279.pdf: 571700 bytes, checksum: 8c5b7a352cd49344a51f6c71fa086c08 (MD5) Previous issue date: 2004-10-22 en
dc.language.iso en_US en
dc.publisher American Society for Biochemistry and Molecular Biology en
dc.subject Telomere/ultrastructure en
dc.subject Telomere/metabolism en
dc.subject Phosphorylation en
dc.subject DNA/chemistry en
dc.title Phosphorylation of H2AX at short telomeres in T cells and fibroblasts en
dc.type Article en

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