Short telomeres induce a DNA damage response in Saccharomyces cerevisiae

Embargo until
Date
2003-03
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Cell Biology
Abstract
Telomerase-deficient Saccharomyces cerevisiae cells show a progressive decrease in telomere length. When grown for several days in log phase, the tlc1Delta cells initially display wild-type growth kinetics with subsequent loss of growth potential after which survivors are generated via RAD52-dependent homologous recombination. We found that chromosome loss in these telomerase-deficient cells only increased after a significant decline in growth potential of the culture. At earlier stages of growth, as the telomerase-deficient cells began to show loss of growth potential, the cells arrested in G2/M and showed RNR3 induction and Rad53p phosphorylation. These responses were dependent on RAD24 and MEC1, suggesting that short telomeres are recognized as DNA damage and signal G2/M arrest.
Description
Keywords
Telomere/metabolism, Telomerase/metabolism, Telomerase/genetics, DNA Damage, Cell Cycle Proteins/metabolism, Cell Cycle/physiology
Citation
IJipma, Arne S. and Carol W. Greider. "Short telomeres induce a DNA damage response in Saccharomyces cerevisiae" Molecular Biology of the Cell 14 (987-1001) (2003 March). Article originally published: http://www.molbiolcell.org/cgi/content/full/14/3/987
Collections