Identification of two RNA-binding proteins associated with human telomerase RNA
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Date
2000-03
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Cell Biology
Abstract
Telomerase plays a crucial role in telomere maintenance in vivo. To understand telomerase regulation, we have been characterizing components of the enzyme. To date several components of the mammalian telomerase holoenzyme have been identified: the essential RNA component (human telomerase RNA [hTR]), the catalytic subunit human telomerase reverse transcriptase (hTERT), and telomerase-associated protein 1. Here we describe the identification of two new proteins that interact with hTR: hStau and L22. Antisera against both proteins immunoprecipitated hTR, hTERT, and telomerase activity from cell extracts, suggesting that the proteins are associated with telomerase. Both proteins localized to the nucleolus and cytoplasm. Although these proteins are associated with telomerase, we found no evidence of their association with each other or with telomerase-associated protein 1. Both hStau and L22 are more abundant than TERT. This, together with their localization, suggests that they may be associated with other ribonucleoprotein complexes in cells. We propose that these two hTR-associated proteins may play a role in hTR processing, telomerase assembly, or localization in vivo.
Description
Keywords
Molecular Sequence Data, RNA-Binding Proteins/metabolism, RNA/metabolism, Biological Transport, Amino Acid Sequences, Telomerase/metabolism
Citation
Le, Siyan, Rolf Sternglanz, and Carol W. Greider. "Identification of two RNA-binding proteins associated with human telomerase RNA" Molecular Biology of the Cell 11 (2000 March): 999-1010. Originally published: http://www.molbiolcell.org/cgi/content/full/11/3/999