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dc.contributor.authorGreider, Carol W.
dc.contributor.authorHemann, Michael T.
dc.date.accessioned2009-11-05T22:21:48Z
dc.date.available2009-11-05T22:21:48Z
dc.date.issued1999-10-15
dc.identifier.citationHemann, Michael T. and Carol W. Greider. "G-strand overhangs on telomeres in telomerase-deficient mouse cells" Nucleic Acids Research 27 (20) (1999 October 15): 3964-3969. Originally published: http://nar.oxfordjournals.org/cgi/content/full/27/20/3964en_US
dc.identifier.urihttp://jhir.library.jhu.edu/handle/1774.2/33544
dc.description.abstractTelomeres of eukaryotic chromosomes contain 3' overhangs which are thought to be essential for the maintenance of proper chromosome end structure and function. We examined the requirement for telomerase activity for the generation of these G-strand overhangs in mammalian cells. Using non-denaturing in-gel hybridization to both tissue and cultured cells from mice deficient for the telomerase RNA component, we found that G-strand overhangs exist in the absence of telomerase activity. Quantitation of overhang signal intensity showed no significant reduction in telomerase-deficient cells relative to wild-type. These results support a telomerase-independent mechanism for generating G-strand overhangsen_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.subjectIn Situ Hybridizationen_US
dc.subjectElectrophoresis, Gel, Pulse-Fielden_US
dc.subjectTelomerase/deficiencyen_US
dc.subjectTelemorase/deficiencyen_US
dc.titleG-strand overhangs on telomeres in telomerase-deficient mouse cellsen_US
dc.typeArticleen_US


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