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dc.contributor.authorGreider, Carol W.
dc.contributor.authorHemann, Michael T.
dc.date.accessioned2009-11-05T22:21:48Z
dc.date.available2009-11-05T22:21:48Z
dc.date.issued1999-10-15
dc.identifier.citationHemann, Michael T. and Carol W. Greider. "G-strand overhangs on telomeres in telomerase-deficient mouse cells" Nucleic Acids Research 27 (20) (1999 October 15): 3964-3969. Originally published: http://nar.oxfordjournals.org/cgi/content/full/27/20/3964en
dc.identifier.urihttp://jhir.library.jhu.edu/handle/1774.2/33544
dc.description.abstractTelomeres of eukaryotic chromosomes contain 3' overhangs which are thought to be essential for the maintenance of proper chromosome end structure and function. We examined the requirement for telomerase activity for the generation of these G-strand overhangs in mammalian cells. Using non-denaturing in-gel hybridization to both tissue and cultured cells from mice deficient for the telomerase RNA component, we found that G-strand overhangs exist in the absence of telomerase activity. Quantitation of overhang signal intensity showed no significant reduction in telomerase-deficient cells relative to wild-type. These results support a telomerase-independent mechanism for generating G-strand overhangsen
dc.language.isoen_USen
dc.publisherOxford University Pressen
dc.subjectIn Situ Hybridizationen
dc.subjectElectrophoresis, Gel, Pulse-Fielden
dc.subjectTelomerase/deficiencyen
dc.subjectTelemorase/deficiencyen
dc.titleG-strand overhangs on telomeres in telomerase-deficient mouse cellsen
dc.typeArticleen


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