G-strand overhangs on telomeres in telomerase-deficient mouse cells

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dc.contributor.author Greider, Carol W.
dc.contributor.author Hemann, Michael T.
dc.date.accessioned 2009-11-05T22:21:48Z
dc.date.available 2009-11-05T22:21:48Z
dc.date.issued 1999-10-15
dc.identifier.citation Hemann, Michael T. and Carol W. Greider. "G-strand overhangs on telomeres in telomerase-deficient mouse cells" Nucleic Acids Research 27 (20) (1999 October 15): 3964-3969. Originally published: http://nar.oxfordjournals.org/cgi/content/full/27/20/3964 en
dc.identifier.uri http://jhir.library.jhu.edu/handle/1774.2/33544
dc.description.abstract Telomeres of eukaryotic chromosomes contain 3' overhangs which are thought to be essential for the maintenance of proper chromosome end structure and function. We examined the requirement for telomerase activity for the generation of these G-strand overhangs in mammalian cells. Using non-denaturing in-gel hybridization to both tissue and cultured cells from mice deficient for the telomerase RNA component, we found that G-strand overhangs exist in the absence of telomerase activity. Quantitation of overhang signal intensity showed no significant reduction in telomerase-deficient cells relative to wild-type. These results support a telomerase-independent mechanism for generating G-strand overhangs en
dc.description.provenance Submitted by David Reynolds (davidr@jhu.edu) on 2009-11-05T22:21:48Z No. of bitstreams: 1 NucAcidRes1999.pdf: 334002 bytes, checksum: 438a1fd3795fd044fae20422bae69329 (MD5) en
dc.description.provenance Made available in DSpace on 2009-11-05T22:21:48Z (GMT). No. of bitstreams: 1 NucAcidRes1999.pdf: 334002 bytes, checksum: 438a1fd3795fd044fae20422bae69329 (MD5) Previous issue date: 1999-10-15 en
dc.language.iso en_US en
dc.publisher Oxford University Press en
dc.subject In Situ Hybridization en
dc.subject Electrophoresis, Gel, Pulse-Field en
dc.subject Telomerase/deficiency en
dc.subject Telemorase/deficiency en
dc.title G-strand overhangs on telomeres in telomerase-deficient mouse cells en
dc.type Article en

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