Telomere Shortening and Tumor Formation by Mouse Cells Lacking Telomerase RNA

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Title: Telomere Shortening and Tumor Formation by Mouse Cells Lacking Telomerase RNA
Author: Greider, Carol W.; DePinho, Ronald A.; Lansdorp, Peter M.; Semper, Enrique; Hande, M. Prakash; Lee, Han-Woong; Blasco, Maria A.
Abstract: To examine the role of telomerase in normal and neoplastic growth, the telomerase RNA component (mTR) was deleted from the mouse germline. mTR-/- mice lacked detectable telomerase activity yet were viable for the six generations analyzed. Telomerase-deficient cells could be immortalized in culture, transformed by viral oncogenes, and generated tumors in nude mice following transformation. Telomeres were shown to shorten at a rate of 4.8+/-2.4 kb per mTR-/- generation. Cells from the fourth mTR-/- generation onward possessed chromosome ends lacking detectable telomere repeats, aneuploidy, and chromosomal abnormalities, including end-to-end fusions. These results indicate that telomerase is essential for telomere length maintenance but is not required for establishment of cell lines, oncogenic transformation, or tumor formation in mice.
URI: http://jhir.library.jhu.edu/handle/1774.2/33678
Date: 1997-10-03
Citation: Reprinted from Cell 91, Blasco, Maria A., et al, "Telomere Shortening and Tumor Formation by Mouse Cells Lacking Telomerase RNA", pg. 25-34, copyright 1997 with permission from Elsevier. Original version available at http://www.cell.com
Subject: Telomerase/physiology
RNA/physiology
Neoplasms, Experimental/pathology
Cell Transformation, Neoplastic

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