Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme

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Title: Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme
Author: Greider, Carol W.; Kobayashi, Ryuji; Collins, Kathleen
Abstract: Telomerase is a ribonucleoprotein DNA polymerase that catalyzes the de novo synthesis of telomeric simple sequence repeats. We describe the purification of telomerase and the cloning of cDNAs encoding two protein subunits from the ciliate Tetrahymena. Two proteins of 80 and 95 kDa copurified and coimmunoprecipitated with telomerase activity and the previously identified Tetrahymena telomerase RNA. The p95 subunit specifically cross-linked to a radiolabeled telomeric DNA primer, while the p80 subunit specifically bound to radiolabeled telomerase RNA. At the primary sequence level, the two telomerase proteins share only limited homologies with other polymerases and polymerase accessory factors.
URI: http://jhir.library.jhu.edu/handle/1774.2/33680
Date: 1995-06-02
Citation: Reprinted from Cell 81, Collins, Kathleen, et al, "Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme", pg. 677-686, copyright 1995 with permission from Elsevier. Original version available at http://www.cell.com
Subject: Tetrahymena thermophila/genetics
Ribonucleoproteins/genetics
Protozoan Proteins/genetics
Genes, Protozoan/genetics
DNA Nucleotidylexotransferase/genetics

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