Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme

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dc.contributor.author Greider, Carol W.
dc.contributor.author Kobayashi, Ryuji
dc.contributor.author Collins, Kathleen
dc.date.accessioned 2009-11-30T18:54:32Z
dc.date.available 2009-11-30T18:54:32Z
dc.date.issued 1995-06-02
dc.identifier.citation Reprinted from Cell 81, Collins, Kathleen, et al, "Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme", pg. 677-686, copyright 1995 with permission from Elsevier. Original version available at http://www.cell.com en
dc.identifier.uri http://jhir.library.jhu.edu/handle/1774.2/33680
dc.description.abstract Telomerase is a ribonucleoprotein DNA polymerase that catalyzes the de novo synthesis of telomeric simple sequence repeats. We describe the purification of telomerase and the cloning of cDNAs encoding two protein subunits from the ciliate Tetrahymena. Two proteins of 80 and 95 kDa copurified and coimmunoprecipitated with telomerase activity and the previously identified Tetrahymena telomerase RNA. The p95 subunit specifically cross-linked to a radiolabeled telomeric DNA primer, while the p80 subunit specifically bound to radiolabeled telomerase RNA. At the primary sequence level, the two telomerase proteins share only limited homologies with other polymerases and polymerase accessory factors. en
dc.description.provenance Submitted by David Reynolds (davidr@jhu.edu) on 2009-11-30T18:54:32Z No. of bitstreams: 1 Cell1995.pdf: 2885451 bytes, checksum: 0cfdad8303007e34844075928cf0958e (MD5) en
dc.description.provenance Made available in DSpace on 2009-11-30T18:54:32Z (GMT). No. of bitstreams: 1 Cell1995.pdf: 2885451 bytes, checksum: 0cfdad8303007e34844075928cf0958e (MD5) Previous issue date: 1995-06-02 en
dc.language.iso en_US en
dc.publisher Cell Press en
dc.subject Tetrahymena thermophila/genetics en
dc.subject Ribonucleoproteins/genetics en
dc.subject Protozoan Proteins/genetics en
dc.subject Genes, Protozoan/genetics en
dc.subject DNA Nucleotidylexotransferase/genetics en
dc.title Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme en
dc.type Article en

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