The Telomere Terminal Transferase of Tetrahymena Is a Ribonucleoprotein Enzyme with Two Kinds of Primer Specificity

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dc.contributor.author Blackburn, Elizabeth H.
dc.contributor.author Greider, Carol W.
dc.date.accessioned 2009-11-30T19:08:15Z
dc.date.available 2009-11-30T19:08:15Z
dc.date.issued 1987-12-24
dc.identifier.citation Reprinted from Cell 51, Greider, Carol W. and Elizabeth H. Blackburn, "The Telomere Terminal Transferase of Tetrahymena Is a Ribonucleoprotein Enzyme with Two Kinds of Primer Specificity", pg. 887-898, copyright 1987 with permission from Elsevier. Original version available at http://www.cell.com en
dc.identifier.uri http://jhir.library.jhu.edu/handle/1774.2/33682
dc.description.abstract We have analyzed the de novo telomere synthesis catalyzed by the enzyme telomere terminal transferase (telomerase) from Tetrahymena. Oligonucleotides representing the G-rich strand of telomeric sequences from five different organisms specifically primed the addition of TTGGGG repeats in vitro, suggesting that primer recognition may involve a DNA structure unique to these oligonucleotides. The sequence at the 3' end of the oligonucleotide primer specified the first nucleotide added in the reaction. Furthermore, the telomerase was shown to be a ribonucleoprotein complex whose RNA and protein components were both essential for activity. After extensive purification of the enzyme by a series of five different chromatographic steps, a few small low abundance RNAs copurified with the activity. en
dc.description.provenance Submitted by David Reynolds (davidr@jhu.edu) on 2009-11-30T19:08:15Z No. of bitstreams: 1 Cell1987.pdf: 4434873 bytes, checksum: 7f8b80c086a579e5fc36deda3f5620f0 (MD5) en
dc.description.provenance Made available in DSpace on 2009-11-30T19:08:15Z (GMT). No. of bitstreams: 1 Cell1987.pdf: 4434873 bytes, checksum: 7f8b80c086a579e5fc36deda3f5620f0 (MD5) Previous issue date: 1987-12-24 en
dc.language.iso en_US en
dc.publisher Cell Press en
dc.subject Tetrahymena/enzymology en
dc.subject Ribonucleoproteins/metabolism en
dc.subject Reptetitive Sequences, Nucleic Acid en
dc.subject Oligodeoxyribonucleotides/metabolism en
dc.subject DNA Nucleotidyltransferases/metabolism en
dc.subject DNA Nucleotidylexotransferase/metabolism en
dc.subject DNA/metabolism en
dc.title The Telomere Terminal Transferase of Tetrahymena Is a Ribonucleoprotein Enzyme with Two Kinds of Primer Specificity en
dc.type Article en

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