Localization of aquaporin CHIP in the human eye: implications in the pathogenesis of glaucoma and other disorders of ocular fluid balance

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dc.contributor.author Regan, J W
dc.contributor.author Agre, P
dc.contributor.author Smith, B L
dc.contributor.author Snyder, R W
dc.contributor.author Stamer, W D
dc.date.accessioned 2010-02-17T20:50:39Z
dc.date.available 2010-02-17T20:50:39Z
dc.date.issued 1994-10
dc.identifier.citation Invest Ophthalmol Vis Sci. 1994 Oct;35(11):3867-72 en
dc.identifier.uri http://jhir.library.jhu.edu/handle/1774.2/33798
dc.description.abstract PURPOSE. The existence of integral membrane proteins that serve as selective water channels has been postulated to explain the movement of water across plasma membranes. Aquaporin CHIP (channel-forming integral membrane protein of 28 kd) is the first such channel to be characterized and is abundant in human erythrocytes and a variety of secretory and absorptive epithelia of the rat. Because disturbances in the movement of water characterize several ocular diseases, the distribution of CHIP in the human eye was studied. METHODS. Affinity-purified antibodies against purified CHIP protein were used for the indirect immunofluorescence localization of CHIP in human eye structures. Labeling was confirmed by immunoblot analyses of membrane preparations from eye structures. RESULTS. CHIP immunolabeling was found in the corneal endothelium, the lens epithelium, the nonpigmented epithelium of the ciliary process, the iris epithelium, and the endothelium of the trabecular meshwork and the canal of Schlemm. CONCLUSIONS. The presence of CHIP water channels in the secretory and absorptive tissues of the human eye provides a mechanism for transcellular water movement and may be important for understanding diseases of the eye that involve excess or insufficient movement of ocular fluid such as glaucoma, cataracts, and Fuch's dystrophy. In addition, the existence of CHIP in the outflow pathways of the human eye provides a novel explanation for the movement of water out of the eye. en
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dc.description.provenance Made available in DSpace on 2010-02-17T20:50:39Z (GMT). No. of bitstreams: 1 3867-1.pdf: 1202599 bytes, checksum: d90b788f0092c5a96247d35272c44bba (MD5) Previous issue date: 1994-10 en
dc.language.iso en_US en
dc.publisher Association for Research in Vision and Ophthalmology en
dc.subject Ion Channels/analysis en
dc.subject Glaucoma/etiology en
dc.subject Aquaporins en
dc.subject Anterior Eye Segment/chemistry en
dc.title Localization of aquaporin CHIP in the human eye: implications in the pathogenesis of glaucoma and other disorders of ocular fluid balance en
dc.type Article en

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