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dc.contributor.authorAgre, Peter
dc.contributor.authorAnstee, David J.
dc.contributor.authorSpring, Frances A.
dc.contributor.authorPreston, Gregory M.
dc.contributor.authorSmith, Barbara L.
dc.date.accessioned2010-02-17T20:59:13Z
dc.date.available2010-02-17T20:59:13Z
dc.date.issued1994-09
dc.identifier.citationJ Clin Invest. 1994 September; 94(3): 1043–1049. doi: 10.1172/JCI117418en_US
dc.identifier.urihttp://jhir.library.jhu.edu/handle/1774.2/33802
dc.description.abstractBlood group antigens are structural variants in surface carbohydrate or amino acid polymorphisms on extracellular domains of membrane proteins. The red cell water channel-forming integral protein (Aquaporin CHIP) is a homotetramer with only one N-glycosylated subunit, however no CHIP-associated blood group antigens have yet been identified. Immunoblotting, monosaccharide composition analysis, and selective glycosidase digestions revealed that the CHIP-associated oligosaccharide contains ABH determinants and resembles a band 3-type glycan that cannot be cleaved from intact membranes by Peptide:N-glycosidase F. The molecular structure of the Colton antigens was previously unknown, but CHIP was selectively immunoprecipitated with anti-Coa or anti-Co(b). The DNA sequence from Colton-typed individuals predicted that residue 45 is alanine in the Co(a+b-) phenotype and valine in the Co(a-b+) phenotype. The nucleotide polymorphism corresponds to a PflMI endonuclease digestion site in the DNA from Co(a-b+) individuals. These studies have defined antigens within two blood group systems on CHIP: (a) an ABH-bearing polylactosaminoglycan attached to a poorly accessible site in the native membrane; and (b) the Colton antigen polymorphism which may permit the identification of rare individuals with defective water channel expression.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Clinical Investigationen_US
dc.subjectOligosaccharides/chemistryen_US
dc.subjectIon Channels/chemistryen_US
dc.subjectIon Channels/blooden_US
dc.subjectErythrocyte Membrane/chemistryen_US
dc.subjectAquaporinsen_US
dc.subjectABO Blood-Group System/chemistryen_US
dc.titleHuman red cell aquaporin CHIP. I. Molecular characterization of ABH and Colton blood group antigensen_US
dc.typeArticleen_US


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