Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei

Show full item record

Title: Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei
Author: Kumar, N; King, L S; Agre, P; Maciel, J; Liu, Y; Promeneur, D
Abstract: The malaria parasite can use host plasma glycerol for lipid biosynthesis and membrane biogenesis during the asexual intraerythrocytic development. The molecular basis for glycerol uptake into the parasite is undefined. We hypothesize that the Plasmodium aquaglyceroporin provides the pathway for glycerol uptake into the malaria parasite. To test this hypothesis, we identified the orthologue of Plasmodium falciparum aquaglyceroporin (PfAQP) in the rodent malaria parasite, Plasmodium berghei (PbAQP), and examined the biological role of PbAQP by performing a targeted deletion of the PbAQP gene. PbAQP and PfAQP are 62% identical in sequence. In contrast to the canonical NPA (Asn-Pro-Ala) motifs in most aquaporins, the PbAQP has NLA (Asn-Leu-Ala) and NPS (Asn-Leu-Ser) in those positions. PbAQP expressed in Xenopus oocytes was permeable to water and glycerol, suggesting that PbAQP is an aquaglyceroporin. In P. berghei, PbAQP was localized to the parasite plasma membrane. The PbAQP-null parasites were viable; however, they were highly deficient in glycerol transport. In addition, they proliferated more slowly compared with the WT parasites, and mice infected with PbAQP-null parasites survived longer. Taken together, these findings suggest that PbAQP provides the pathway for the entry of glycerol into P. berghei and contributes to the growth of the parasite during the asexual intraerythrocytic stages of infection. In conclusion, we demonstrate here that PbAQP plays an important role in the blood-stage development of the rodent malaria parasite during infection in mice and could be added to the list of targets for the design of antimalarial drugs.
URI: http://jhir.library.jhu.edu/handle/1774.2/33890
Date: 2007-02-13
Citation: Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2211-6. Epub 2007 Feb 6. http://www.pnas.org/content/104/7/2211.abstract
Subject: Plasmodium berghei/chemistry
Erythrocytes/parasitology
Aquaglyceroporins/metabolism

Files in this item

Files Size Format Download
PNAS-2007-Promeneur-2211-6.pdf 1.138Mb application/pdf Download

The following license files are associated with this item:

This item appears in the following Collection(s)

Show full item record