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dc.contributor.authorSmith, B L
dc.contributor.authorDenker, B. M.
dc.contributor.authorAgre, P
dc.contributor.authorKuhajda, F. P.
dc.date.accessioned2010-03-04T20:57:00Z
dc.date.available2010-03-04T20:57:00Z
dc.date.issued1988-10-25
dc.identifier.citationJ Biol Chem. 1988 Oct 25;263(30):15634-42. http://www.jbc.org/content/263/30/15634.longen_US
dc.identifier.urihttp://jhir.library.jhu.edu/handle/1774.2/33905
dc.description.abstractA novel Mr 28,000 integral membrane protein ("28kDa") was identified in human erythrocytes and found entirely associated with the Triton X-100 insoluble membrane skeletons. Antibodies to 28kDa reacted strongly on immunoblots with 28kDa and a diffuse region of Mr 35,000-60,000 ("HMW-28kDa"). Selective proteolytic digestions of membranes demonstrated that HMW-28kDa has an extracellular domain, and both 28kDa and HMW-28kDa have intracellular domains. 28kDa and HMW-28kDa were purified to homogeneity. Quantitative immunoblots indicate that each erythrocyte contains 120,000-160,000 copies of 28kDa. Two-dimensional iodopeptide maps of 28kDa and HMW-28kDa were nearly identical; peptide-N-glycosidase digestion of purified HMW-28kDa demonstrated that it is the N-glycosylated form of 28kDa. When concentrated, 28kDa formed a series of larger oligomers which were stable in sodium dodecyl sulfate. Of several nonerythroid tissues studied with anti-28kDa immunoblots, only kidney displayed immunoreactive 28kDa. Purified rat kidney 28kDa was nearly identical to rat erythrocyte 28kDa when compared by two-dimensional iodopeptide mapping. Immunohistochemical staining of human kidney with anti-28kDa demonstrated prominent staining over the apical brush borders of proximal convoluted tubules. A novel integral membrane protein has been purified from erythrocyte and kidney membranes. This new protein may play a role in linkage of the membrane skeleton to the lipid bilayer.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_US
dc.subjectMembrane Proteins/analysisen_US
dc.subjectErythrocytes/analysisen_US
dc.subjectKidney Tubules/analysisen_US
dc.titleIdentification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubulesen_US
dc.typeArticleen_US


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