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dc.contributor.authorAgre, Peter
dc.contributor.authorZeidel, Mark L.
dc.contributor.authorRaben, Daniel
dc.contributor.authorNielsen, Søren
dc.contributor.authorBaumgarten, Ruben
dc.contributor.authorSmith, Barbara L.
dc.date.accessioned2010-03-04T22:25:05Z
dc.date.available2010-03-04T22:25:05Z
dc.date.issued1993-10
dc.identifier.citationJ Clin Invest. 1993 October; 92(4): 2035–2041. doi: 10.1172/JCI116798en_US
dc.identifier.urihttp://jhir.library.jhu.edu/handle/1774.2/33913
dc.description.abstractMajor phenotypic changes occur in red cell membranes during the perinatal period, but the underlying molecular explanations remain poorly defined. Aquaporin CHIP, the major erythroid and renal water channel, was studied in perinatal rats using affinity-purified anti-CHIP IgG for immunoblotting, flow cytometry, and immunofluorescence microscopy. CHIP was not detected in prenatal red cells but was first identified in circulating red cells on the third postnatal day. Most circulating red cells were positive for CHIP by the seventh postnatal day, and this proportion rose to nearly 100% by the 14th day. The ontogeny of red cell CHIP correlated directly with acquisition of osmotic water permeability and inversely with Arrhenius activation energy. Only minor alterations in the composition of red cell membrane lipids occurred at this time. Immunohistochemical analysis of perinatal kidneys demonstrated a major induction of CHIP in renal proximal tubules and descending thin limbs at birth, coincident with the development of renal concentration mechanisms. Therefore, water channels are unnecessary for oxygen delivery or survival in the prenatal circulation, however CHIP may confer red cells with the ability to rehydrate rapidly after traversing the renal medulla, which becomes hypertonic after birth.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Clinical Investigationen_US
dc.subjectMembrane Proteins/blooden_US
dc.subjectMembrane Proteins/biosynthesisen_US
dc.subjectKidney/metabolismen_US
dc.subjectErythrocyte Membrane/metabolismen_US
dc.subjectAquaporinsen_US
dc.subjectAging/metabolismen_US
dc.titleConcurrent expression of erythroid and renal aquaporin CHIP and appearance of water channel activity in perinatal ratsen_US
dc.typeArticleen_US


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