Polymorphism in the Mr 32,000 Rh protein purified from Rh(D)-positive and -negative erythrocytes

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dc.contributor.author Agre, P
dc.contributor.author Smith, B L
dc.contributor.author Saboori, A M
dc.date.accessioned 2010-03-10T21:36:49Z
dc.date.available 2010-03-10T21:36:49Z
dc.date.issued 1988-06
dc.identifier.citation Proc Natl Acad Sci U S A. 1988 Jun;85(11):4042-5. http://www.pnas.org/content/85/11/4042.abstract en
dc.identifier.uri http://jhir.library.jhu.edu/handle/1774.2/33956
dc.description.abstract A Mr 32,000 integral membrane protein has previously been identified on erythrocytes bearing the Rh(D) antigen and is thought to contain the antigenic variations responsible for the different Rh phenotypes. To study it on a biochemical level, a simple large-scale method was developed to purify the Mr 32,000 Rh protein from multiple units of Rh(D)-positive and -negative blood. Erythrocyte membrane vesicles were solubilized in NaDodSO4, and a tracer of immunoprecipitated 125I surface-labeled Rh protein was added. The Rh protein was purified to homogeneity by hydroxylapatite chromatography followed by preparative NaDodSO4/PAGE. Approximately 25 nmol of pure Rh protein was recovered from each unit of Rh(D)-positive and -negative blood. Rh protein purified from both Rh phenotypes appeared similar by one-dimensional NaDodSO4/PAGE, and the N-terminal amino acid sequences for the first 20 residues were identical. Rh proteins purified from Rh(D)-positive and -negative blood were compared by two-dimensional iodopeptide mapping after 125I-labeling and alpha-chymotrypsin digestion. The peptide maps were very similar; however, at least two additional iodopeptides were consistently noted in the Rh proteins purified from Rh(D)-positive erythrocytes. These data indicate that a similar core Rh protein (or group of related proteins) exists in both Rh(D)-positive and -negative erythrocytes, and the Rh proteins from erythrocytes with different Rh phenotypes contain distinct structural polymorphisms. en
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dc.description.provenance Made available in DSpace on 2010-03-10T21:36:49Z (GMT). No. of bitstreams: 1 PNAS-1988-Saboori-4042-5.pdf: 1104357 bytes, checksum: 6a7496ad3499a5e652037ba2eee99b25 (MD5) Previous issue date: 1988-06 en
dc.language.iso en_US en
dc.publisher National Academy of Sciences en
dc.subject Rh-Hr Blood-Group System/genetics en
dc.subject Membrane Glycoproteins/blood en
dc.subject Erythrocyte Membrane/analysis en
dc.title Polymorphism in the Mr 32,000 Rh protein purified from Rh(D)-positive and -negative erythrocytes en
dc.type Article en

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