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Bacterial expression strategies for several Sus scrofa diacylglycerol kinase alpha constructs: solubility challenges.
(Nature Publishing Group, 2013-04-05)
We pursued several strategies for expressing either full-length Sus scrofa diacylglycerol kinase (DGK) alpha or the catalytic domain (alphacat) in Escherichia coli. Alphacat could be extracted, refolded, and purified from ...