• Login
    View Item 
    •   JScholarship Home
    • JHU Libraries Open Access Fund
    • 2012/2013 Recipients
    • View Item
    •   JScholarship Home
    • JHU Libraries Open Access Fund
    • 2012/2013 Recipients
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    LINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransduction

    Thumbnail
    View/Open
    Research Article (1.826Mb)
    Date
    2013-01-18
    Author
    Chambliss, Allison B.
    Khatau, Shyam B.
    Erdenberger, Nicholas
    Robinson, D. Kyle
    Hodzic, Didier
    Longmore, Gregory D.
    Wirtz, Denis
    Metadata
    Show full item record
    Abstract
    Cells continuously sense and respond to external mechanical forces through their cytoskeleton. Here we show that only a small subset of actin fibers, those forming the perinuclear actin cap that wraps around the nucleus, form in response to low physiological mechanical stresses in adherent fibroblasts. While conventional basal stress fibers form only past a threshold shear stress of 0.5 dyn/cm(2), actin-cap fibers are formed at shear stresses 50 times lower and orders-of-magnitude faster than biochemical stimulation. This fast differential response is uniquely mediated by focal adhesion protein zyxin at low shear stress and actomyosin fibers of the actin cap. We identify additional roles for lamin A/C of the nuclear lamina and linkers of nucleus to cytoskeleton (LINC) molecules nesprin2giant and nesprin3, which anchor actin cap fibers to the nucleus. These results suggest an interconnected physical pathway for mechanotransduction, from the extracellular milieu to the nucleus.
    URI
    http://jhir.library.jhu.edu/handle/1774.2/36712
    Collections
    • 2012/2013 Recipients

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of JScholarshipCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV