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dc.contributor.authorChambliss, Allison B.
dc.contributor.authorKhatau, Shyam B.
dc.contributor.authorErdenberger, Nicholas
dc.contributor.authorRobinson, D. Kyle
dc.contributor.authorHodzic, Didier
dc.contributor.authorLongmore, Gregory D.
dc.contributor.authorWirtz, Denis
dc.date.accessioned2014-03-28T13:56:47Z
dc.date.available2014-03-28T13:56:47Z
dc.date.issued2013-01-18
dc.identifier.citationdoi: 10.1038/srep01087en_US
dc.identifier.issn2045-2322
dc.identifier.urihttp://jhir.library.jhu.edu/handle/1774.2/36712
dc.descriptionPMC3548190en_US
dc.description.abstractCells continuously sense and respond to external mechanical forces through their cytoskeleton. Here we show that only a small subset of actin fibers, those forming the perinuclear actin cap that wraps around the nucleus, form in response to low physiological mechanical stresses in adherent fibroblasts. While conventional basal stress fibers form only past a threshold shear stress of 0.5 dyn/cm(2), actin-cap fibers are formed at shear stresses 50 times lower and orders-of-magnitude faster than biochemical stimulation. This fast differential response is uniquely mediated by focal adhesion protein zyxin at low shear stress and actomyosin fibers of the actin cap. We identify additional roles for lamin A/C of the nuclear lamina and linkers of nucleus to cytoskeleton (LINC) molecules nesprin2giant and nesprin3, which anchor actin cap fibers to the nucleus. These results suggest an interconnected physical pathway for mechanotransduction, from the extracellular milieu to the nucleus.en_US
dc.description.sponsorshipJH Libraries Open Access Funden_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofseriesScientific reports;v. 3, rpt. 1087 -
dc.subjectNuclear Proteinsen_US
dc.subjectNerve Tissue Proteinsen_US
dc.subjectMembrane Proteinsen_US
dc.subjectMechanotransduction, Cellularen_US
dc.subjectCell Nucleusen_US
dc.subjectActinsen_US
dc.titleLINC-anchored actin cap connects the extracellular milieu to the nucleus for ultrafast mechanotransductionen_US
dc.typeArticleen_US


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