Decoupling nucleosome recognition from DNA binding dramatically alters the properties of the Chd1 chromatin remodeler
Embargo until
Date
2012-12-28
Journal Title
Journal ISSN
Volume Title
Publisher
Oxford University Press
Abstract
Chromatin remodelers can either organize or disrupt
nucleosomal arrays, yet the mechanisms specifying
these opposing actions are not clear. Here, we show
that the outcome of nucleosome sliding by Chd1
changes dramatically depending on how the chromatin
remodeler is targeted to nucleosomes. Using a
Chd1–streptavidin fusion remodeler, we found that
targeting via biotinylated DNA resulted in directional
sliding towards the recruitment site, whereas targeting
via biotinylated histones produced a distribution of
nucleosome positions. Remarkably, the fusion remodeler
shifted nucleosomes with biotinylated
histones up to 50bp off the ends of DNA and was
capable of reducing negative supercoiling of
plasmids containing biotinylated chromatin, similar
to remodelling characteristics observed for SWI/
SNF-type remodelers. These data suggest that
forming a stable attachment to nucleosomes via
histones, and thus lacking sensitivity to extranucleosomal
DNA, seems to be sufficient for allowing
a chromatin remodeler to possess SWI/SNF-like disruptive
properties.
Description
PMC3561990
Keywords
Saccharomyces cerevisiae Proteins, Nuclesomes, DNA-Binding Proteins, DNA, Chromatin Assembly and Disassembly
Citation
doi:10.1093/nar/gks1440