Characterization of Sac7 as a Negative Regulator of the Rho1-mediated Clathrin Independent Endocytic Pathway in Saccharomyces cerevisiae
Peck, Matthew Richard
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Although it had, until recently, been thought that clathrin-mediated endocytosis was the sole mechanism for endocytosis in Saccharomyces cerevisiae, a clathrin-independent pathway that utilizes the small GTPase, Rho1, was recently discovered. Small GTPases are positively regulated by GTP exchange factors, or GEFs, and negatively regulated by GTPase activing proteins, or GAPs. Relatively little is known about Sac7, the primary GAP of this pathway. This study presents the finding of multiple proteins that physically interact with Sac7 via a yeast two-hybrid screen and preliminary evidence regarding the potential roles these proteins may play in the clathrin-independent endocytic pathway.