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dc.contributor.advisorRay, Stuart C.
dc.creatorLaskey, Sarah B.
dc.date.accessioned2017-04-19T12:24:26Z
dc.date.available2017-04-19T12:24:26Z
dc.date.created2016-12
dc.date.issued2016-06-16
dc.date.submittedDecember 2016
dc.identifier.urihttp://jhir.library.jhu.edu/handle/1774.2/40297
dc.description.abstractAlthough great progress has been achieved in the fight against HIV and AIDS, an epidemic continues to rage worldwide. Alongside public health and clinical efforts, scientific research plays a major role in this fight; ongoing studies focus on improving treatment options, designing a vaccine, and developing a cure. The first study described in this dissertation is a critique of research and analysis methods commonly used to characterize the latent HIV reservoir, which is considered a barrier to the cure of HIV infection. This project highlights the importance of understanding molecular biology tools and considering their inherent limitations when interpreting results. The second study presented here comprises a quantitative evaluation of the efficacy of dolutegravir, an antiretroviral drug that has emerged recently as one of our best weapons for controlling HIV infection. Results show that the high efficacy of dolutegravir in combination with other antiretroviral drugs, as well as the high genetic barrier to viral evolution of dolutegravir resistance, are consistent with the tremendous clinical success of this drug. The grand conclusion of this dissertation is that much work remains to be done. We continue to discover safer, more effective tools for the treatment of HIV infection, which may be seen as a silver lining given how far we appear to be from achieving a cure.
dc.format.mimetypeapplication/pdf
dc.publisherJohns Hopkins University
dc.subjectHIV
dc.subjectantiretroviral therapy
dc.subjectclonal expansion
dc.subjectlatency
dc.subjectlatent reservoir
dc.subjectdolutegravir
dc.subjectPCR
dc.subjectclonal prediction score
dc.titleAttacking the HIV Epidemic From All Sides: Optimization of PCR Strategies to Measure Clonal Expansion and Quantitative Evaluation of Dolutegravir
dc.typeThesis
thesis.degree.disciplineImmunology
thesis.degree.grantorJohns Hopkins University
thesis.degree.grantorSchool of Medicine
thesis.degree.levelDoctoral
thesis.degree.namePh.D.
dc.date.updated2017-04-19T12:24:26Z
dc.type.materialtext
thesis.degree.departmentBiomedical Engineering
dc.contributor.committeeMemberSiliciano, Robert F.
dc.contributor.committeeMemberBlankson, Joel N.
dc.contributor.committeeMemberTimp, Winston
dc.publisher.countryUSA
dc.creator.orcid0000-0002-7402-3702


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