Implementing in vitro screening tools for evaluating novel mRNA-nanoparticulate formulations
Vigil, Toriana Nichole
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3D spheroids of immortalized cell lines and primary human cells were utilized to examine the structure-function relationship of lipid nanoparticles (LNPs). Establishing a rigorous procedure for HepG2 and Hep3b spheroids included determination of appropriate seeding densities and media conditions for LNP transfection. Experimentation with standard and non-standard LNP formulations highlighted significant variation with cell and culture type, thus suggesting that choosing appropriately is of the utmost importance. Furthermore, it was noted that LNP performance should be determined via evaluation in multiple metrics since potency and maximum expression results did not always align. An in vitro to in vivo correlation with LNP transfection is ongoing.