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dc.contributor.advisorEwald, Andrew J
dc.creatorGentz, Melissa L
dc.date.accessioned2021-06-25T20:02:44Z
dc.date.available2021-06-25T20:02:44Z
dc.date.created2021-05
dc.date.issued2021-05-06
dc.date.submittedMay 2021
dc.identifier.urihttp://jhir.library.jhu.edu/handle/1774.2/64285
dc.description.abstractEpithelial-to-mesenchymal transition (EMT) and its reversed process, mesenchymal-to- epithelial transition (MET) remain a topic of debate among the cancer research community. Here, we sought to isolate two novel cell lines from the basal-like mouse models (ROSAmT/mG C3(1)- Tag and C3(1)-Tag) of triple-negative breast cancer (TNBC) to investigate the effects of 2D and 3D culture methods on the expression of epithelial and mesenchymal phenotypes. We demonstrated that co-expression of E-cadherin and vimentin is present in both cell lines when cultured in 2D. Our data also suggests that when embedded in 3D matrices including Rat tail collagen 1 and Matrigel, TGF􏰚 signaling induces spheroid invasion. However, it may play a role in inhibiting colony formation in Matrigel. Taken together, my data reveal that C3(1)-TagM and C3(1)-Tag cell lines may provide researchers with an alternate platform to research hybrid EMT.
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherJohns Hopkins University
dc.subjectC3(1)-Tag, triple negative breast cancer, TNBC, basal breast cancer, EMT, MET, vimentin, TGFbeta
dc.titleIsolation and characterization of two basal breast cancer model cell lines
dc.typeThesis
thesis.degree.disciplineBiochemistry
thesis.degree.grantorJohns Hopkins University
thesis.degree.grantorBloomberg School of Public Health
thesis.degree.levelMasters
thesis.degree.nameSc.M.
dc.date.updated2021-06-25T20:02:44Z
dc.type.materialtext
thesis.degree.departmentBiochemistry, Cellular and Molecular Biology
local.embargo.lift2022-05-01
local.embargo.terms2022-05-01
dc.contributor.committeeMemberKavran, Jennifer M
dc.publisher.countryUSA
dc.creator.orcid0000-0002-1708-6416


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