3D VISCOELASTIC ENVIRONMENT REGULATES RESPONSE OF MACROPHAGES

Embargo until
2026-05-01
Date
2022-05-06
Journal Title
Journal ISSN
Volume Title
Publisher
Johns Hopkins University
Abstract
Macrophages are innate immune cells with multiple physiological roles in the body. They respond to both biochemical and biophysical cues from the viscoelastic tissue environment and are highly plastic in changing their phenotypes and corresponding behaviors. Although many studies are focusing on macrophages response to stimuli of soluble factors, how matrix properties regulate their response and function is largely unknown. Here, in this thesis we proposed a hydrogel system that mimics the viscoelasticity of native tissue and cultured bone marrow-derived macrophages (BMDM) in 3D environments to study their behaviors in response to matrix mechanics. We found that under proinflammatory factors (IFN-γ+LPS) stimulation, macrophages expressed different levels of inducible nitric oxide synthase (iNOS), which is a key enzyme in the macrophage inflammatory response. This indicates that 3D viscoelastic environments regulate the inflammatory response of macrophages and faster stress relaxation of matrix downregulates its activation. This finding has potential implications in future studies in macrophages-related immune diseases.
Description
Keywords
macrophage, 3D environment, viscoelastic, immune disease
Citation