A STUDY OF BACTERIAL TRANSLATION AT CODON RESOLUTION USING RIBOSOME PROFILING
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Date
2019-12-06
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Johns Hopkins University
Abstract
Ribosome profiling has pushed the boundary of how translation is studied by illuminating every step of the translation cycle at the genome scale. First developed by Nick Ingolia and Jonathan Weismann, ribosome profiling is now widely used in both bacterial and eukaryotic studies. However, development of the method in bacteria has not achieved the level of refinement seen in yeast and mammalian ribosome profiling. This thesis focuses on analyzing the current methodology in bacteria to understand its strengths and shortcomings and developing improvements in both how libraries are prepared and how the data is analyzed. This thesis will also focus on implementing these improvements to understand events that influence translation elongation as well as how ribosome profiling can be used to identify new genes.
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Keywords
Ribosome, translation, next generation sequencing, ribosome profiling, E. coli, ORF, gene annotation