ARSENIC SPECIATION WITHIN THE MAMMALIAN METABOLISM
Embargo until
2022-05-01
Date
2020-05-11
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Publisher
Johns Hopkins University
Abstract
Inorganic arsenic (iAs) is a ubiquitous element in the environment- it innately exists within the earth’s crust and mobilizes into the atmosphere, soil, water, and within organisms [1]. Information on arsenic speciation within tissues is limited, although speciation is vital given that each arsenic species has different qualitative and quantitative differences in toxicity [2]. The goal of this thesis is to (1) provide a non-systematic literature review to compare the current state of literature and define the gaps in mammalian arsenic speciation and note arsenic metabolism within the lungs, liver, and spleen and to (2) develop arsenic extraction and analysis methods to assess arsenic in lung, spleen, and liver tissues of male and female C57BL6 mice exposed to 0, and 1000 ppb of sodium (meta) arsenite in drinking water. Arsenic species iAsIII, iAsV, MMA, and DMA were examined through use of high-performance liquid chromatography joined with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) within spiked mammalian tissues. Arsenic species were detected during a preliminary assessment of 1000 ppb dosed spleen and lung. This research evaluates a method for arsenic speciation in different tissues. The use of these method in follow-up studies will provide insight for the potential biochemical pathways, toxicological mechanisms, and allow for future inferences about arsenic species in mammalian tissue.
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Keywords
Arsenic species, Arsenic Speciation, Arsenic Metabolism